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. 2020 Nov 24;11:493. doi: 10.1186/s13287-020-02019-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — CD34+ CD43+ cells derived from human ESCs generate T-lymphocytes with a broad T cell receptor (TCR) repertoire. a Representative flow cytometry analysis of CD5 and CD7 expression in ESC-derived CD34+ CD43+ cells after 22 days of T cell differentiation on OP9/DLL1 co-culture. b Representative flow cytometry analysis of CD4, CD8, and CD3 expression in ESC-derived CD34+ CD43+ cells at 22 days of T cell differentiation on OP9/DLL1 co-culture. c The frequency of CD3+ cells in CD4+CD8+ double-positive T cells on day 22 and day 27. Values represent mean ± SD (n = 3). d TCR-Vβ deep sequencing showed the diversity of rearranged TCR genes in ESC-derived CD4+ CD8+ CD3+ T cells at day 27 of differentiation