Figure 4.

Hit summary from NPC and AIL compound sets. AlphaLISA and TruHits assays were performed against the NPC and AIL compound sets (3384 compounds total). Potential hits in both the AlphaLISA and TruHits assays were defined as having an IC₅₀ ≥ 50 μM and a maximum response less than or equal to −50%. Of those hits, 89 were found to be unique to the AlphaLISA assay, and those curves were visually inspected to identify 25 high-quality hits for cherry-picking. Low-quality hits have TruHits response curves that satisfy the conditions for a “potential hit” but do not significantly diverge from the AlphaLISA data. High-quality hits have minimal TruHits response which do significantly diverge from the AlphaLISA response curve.