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Compound 4t is a reversible and competitive inhibitor of mPTPB with pNPP as a substrate. Lineweaver–Burk plot for 4t-mediated mPTPB inhibition. Compound 4t concentrations were 0 (●), 10 (○), and 20 (▼) nM, respectively. The Ki value of 2.7 ± 0.2 nM was determined from three independent measurements.
