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. Author manuscript; available in PMC: 2020 Nov 25.
Published in final edited form as: J Med Chem. 2020 Aug 21;63(17):9212–9227. doi: 10.1021/acs.jmedchem.0c00302

Figure 6.

Figure 6.

Cellular efficacy of the mPTPB inhibitor 4t. Raw 264.7 cells were transfected with pcN-HA control vector (lanes 1–6) or pcN-HA-mPTPB (lanes 7–12) to create stable cell lines.18 Raw264.7 cells expressing mPTPB exhibited decreased IFN-γ-stimulated ERK1/2 and p38 activation and increased AKT activity compared to control vector cells. Cellular activity of compound 4t was evaluated by treating it with both cell lines. Compound 4t was able to block mPTPB-mediated cellular signaling by restoring ERK1/2 and p38 activity and inhibiting AKT activation by mPTPB, whereas no effect on control vector-transfected cells was observed as shown by the blots above.