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Kinetic Parameters of the Wild-Type and Mutant mPTPBs with pNPP as a Substratea
| enzyme | Km (in mM) | kcat (s−1) |
|---|---|---|
| wild type | 3.58 ± 0.11 | 4.37 ± 0.12 |
| I203A | 2.73 ± 0.09 | 1.61 ± 0.10 |
| I207A | 4.85 ± 0.17 | 4.94 ± 0.19 |
| I207K | 4.53 ± 0.05 | 1.96 ± 0.02 |
| F161S | 4.47 ± 0.27 | 0.30 ± 0.02 |
| K164I | NA | NA |
| D165G | NA | NA |
Kinetics measured at pH = 7.0, in 50.0 mM 3,3-dimethylglutarate, 1 mM EDTA, 0.15 M NaCl buffer at room temperature. NA = showed no measurable phosphatase activity.
