KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| E. Coli BL21(DE3) Chemically Competent Cells | Millipore Sigma | CMC0014-20X40UL |
| NEB® Turbo Competent E. coli (High Efficiency) | New England Biolabs | C2984H |
| Chemicals, Peptides, and Recombinant Proteins | ||
| AcTEV Protease | Fisher Scientific | 12-575-015 |
| Ribonuclease A from bovine pancreas | Millipore Sigma | R6513 |
| cOmplete™ Protease Inhibitor Cocktail | Roche | 11697498001 |
| Glucose Oxidase from Aspergillus niger | Sigma-Aldrich | G2133 |
| Catalase from bovine liver | Sigma-Aldrich | C3155 |
| IGEPAL® CA-630 | Sigma-Aldrich | I8896 |
| His-Trap FF Crude, 5 mL | GE Healthcare | 17528601 |
| Cy3 NHS Ester | GE Healthcare | PA13101 |
| Cy5 NHS Ester | GE Healthcare | PA15100 |
| Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester) | Thermo Scientific | A20004 |
| Abberior STAR 635P | Abberior | ST635P |
| Janelia Fluor® 646 HaloTag® Ligand | Promega | GA112A |
| RNase Inhibitor, Murine | New England Biolabs | M0314L |
| Lipofectamine™ 3000 Transfection Reagent | Thermo Scientific | L3000001 |
| Sodium (meta)arsenite (>90%) | Millipore Sigma | S7400-100G |
| Experimental Models: Cell Lines | ||
| Human: SH-SY5Y | ATCC | CRL-2266 |
| Oligonucleotides | ||
| Biotin-18mer: 5’- /biotin/rUrGrG rCrGrA rCrGrG rCrArG rCrGrA rGrGrC/3AmMO/ -3’ | Niaki et al., 2020; IDT | N/A |
| U50-18mer: 5’- /5AmMC6/rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrG rCrCrU rCrGrC rUrGrC rCrGrU rCrGrC rCrA -3’ | Niaki et al., 2020; IDT | N/A |
| U40: 5’- rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rUrUrU rU/3AmMO/ -3’ | Niaki et al., 2020; IDT | N/A |
| Recombinant DNA | ||
| pTHMT/FUSWT (encoding 6xHis-MBP-FUSWT) | Niaki et al., 2020 | N/A |
| pTHMT/FUSR244C (encoding 6xHis-MBP-FUSR244C) | Niaki et al., 2020 | N/A |
| pTHMT/FUSR216C (encoding 6xHis-MBP-FUSR216C) | Niaki et al., 2020 | N/A |
| pTHMT/FUSR521G (encoding 6xHis-MBP-FUSR521G) | Niaki et al., 2020 | N/A |
| pTHMT/FUSG156E (encoding 6xHis-MBP-FUSG156E) | Niaki et al., 2020 | N/A |
| pTHMT/FUSG187S (encoding 6xHis-MBP-FUSG187S) | Niaki et al., 2020 | N/A |
| pTHMT/FUSG399V(encoding 6xHis-MBP-FUSG399V) | Niaki et al., 2020 | N/A |
| pTHMT/FUSRBD(encoding 6xHis-MBP-FUSRBD) | Genscript | N/A |
| pTHMT/FUSLCD(encoding 6xHis-MBP-FUSLCD) | Genscript | N/A |
| pTHMT/FUSG156A(encoding 6xHis-MBP-FUSG156A) | Genscript | N/A |
| pTHMT/FUSG156D(encoding 6xHis-MBP-FUSG156D) | Genscript | N/A |
| pTHMT/FUSG156Q(encoding 6xHis-MBP-FUSG156Q) | Genscript | N/A |
| pTHMT/FUSG154E(encoding 6xHis-MBP-FUSG154E) | Genscript | N/A |
| pTHMT/FUSG156P(encoding 6xHis-MBP-FUSG154E) | Genscript | N/A |
| pTHMT/FUSWT-EGFP (encoding 6xHis-MBP-FUSWT-EGFP) | Niaki et al., 2020 | N/A |
| pTHMT/FUSWT-mCherry (encoding 6xHis-MBP-FUSWT-mCherry) | Genscript | N/A |
| pTHMT/FUSR244C-EGFP (encoding 6xHis-MBP-FUSR244C-EGFP) | Genscript | N/A |
| pTHMT/FUSG156E-EGFP (encoding 6xHis-MBP-FUSG156E-EGFP) | Genscript | N/A |
| pFUSWT-GFP_pcDNA3.1(+)-C-eGFP | Genscript | N/A |
| pFUSWT-Halo_piggybac-EF1-Halo | Li et al., 2016; Genscript | N/A |
| pFUSR244C-Halo_piggybac-EF1-Halo | Li et al., 2016; Genscript | N/A |
| pFUSG156E-Halo_piggybac-EF1-Halo | Li et al., 2016; Genscript | N/A |
| Software and Algorithms | ||
| MATLAB and IDL scripts | https://physics.illinois.edu/cplc/software/; This manuscript | |
| NIS-Elements Ar Package | Nikon Inc. | |
| Adobe Photoshop CC | Adobe (https://www.adobe.com/products/photoshop.html) | |
| Prism 7 | GraphPad (https://www.graphpad.com/scientific-software/prism/) | |
| ImageJ (Fiji) | NIH (https://imagej.nih.gov/ij/) | |
| RStudio | RStudio (https://rstudio.com/products/rstudio/download/) | |
| Other | ||
| Nunc™ Lab-Tek™ Chambered Coverglass | Thermo Scientific | 155361 |
| DNA Retardation Gels (6%) | Invitrogen | EC63655BOX |
| Amicon Ultra-0.5 Centrifugal Filter Unit | Millipore | UFC501096 |
| Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL | Thermo Scientific | 89883 |