TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| Burkholderia thailandensis strains | ||
| E264 | Wild type | 5 |
| BD20 | E264 with deletion of btaK | 11 |
| JRK100 | E264 hmqA::cm | This study |
| JRK101 | E264 BTH_II1576::FRT-tmp | This study |
| JRK102 | BD20 hmqA::cm | This study |
| JRK103 | BD20 BTH_II1576::FRT-tmp | This study |
| JRK104 | BD20 glmS1 attn7::Plac; Kmr | This study |
| JRK105 | JRK103 glmS1 attn7::Plac; Kmr | This study |
| JRK106 | JRK103 glmS1 attn7::Plac-hmqL; Kmr | This study |
| Escherichia coli strains | ||
| DH5α | F− ϕ80lacZ ΔM15 Δ(lacZYA-argF)U169 hsdR17(rK− mK+) recA1 endA1 phoA supE44 thi-1 gyrA96 relA1 λ− | Invitrogen |
| JM109 | (traD36 proAB+ lacIq lacZ M15) endA1 recA1 hsdR17(rK− mK+) mcrA supE44 λ− gyrA96 relA1 (lac proAB) | 67 |
| Other strains | ||
| Bacillus subtilis 168 | Wild type | 68 |
| Staphylococcus aureus Newman | Wild type | 57 |
| Pseudomonas aeruginosa PA14 | Wild type | 58 |
| Burkholderia ambifaria HSJ1 | Wild type | 21 |
| Plasmids | ||
| pJRC125 | Suicide plasmid; Tpr | 6 |
| pMCG19 | pEX18Tp-PheS with an hmqA disrupted by a chloramphenicol resistance cassette (hmqA::cm) | This study |
| pTNS2 | Tn7 transposase-expressing helper plasmid; Ampr | 69 |
| pUC18miniTn7T-Plac-malR | Mobilizable mini-Tn7 vector with the lac promoter (Plac) for IPTG-inducible malR expression (used to construct pUC18-mini-Tn7T-Plac-malR); Kmr, Apr | 61 |
| pUC18miniTn7T-Plac-hmqL | pUC18miniTn7T-LAC-Km containing the BTH_II1576 gene (hmqL); Kmr, Apr | This study |
| pME6010 | pVS1-p15A shuttle vector; Tcr | 33 |
| pME6010-hmqL | pME6010 with the B. thailandensis BTH_II1576 (hmqL) gene | This study |