TABLE 4.
Primers used in this study
| Purpose, primer | Sequence (5′ to 3′) |
|---|---|
| Tn5 mutant identification | |
| hmqA F-1 | GATCTGCCATTGCTTTCCGCAACACG |
| hmqA R-1 | TCAGGCCGCTTGCACGTCG |
| hmqF F-1 | GCTGCATCTGAAGAGCATGGAGC |
| hmqF R-1 | CGTGCTCTCTTCGTGATATCCCATCC |
| hmqC F-1 | TCGGCAATGTGCGAAGCAAGGTC |
| hmqC R-1 | GAGCGGATTGTCGGCAACGAC |
| hmqL-Tn5-Tp-F2 | CGTCATGCCCAATGTGCGCTTG |
| hmqL-Tn5-Tp-R2 | GTTGGTTGACGACTGCGCGAAC |
| Constructing pUC18miniTn7T-Plac-hmqL (BTH_II1576) | |
| hmqL-ORF-F-SacI | ATATTAGAGCTCATGAAAAACAACCAAGTCGATG |
| hmqL-ORF-R-HindIII | ATATTAAAGCTTATTCCCGCTTCGTCCGCCAGC |
| Constructing pMCG19 (hmqA::cm) | |
| hmqAfor | ACGAAGCTTCATCTCTTGCCGCAGCTTGAA |
| hmqArev | ACGGTACCGATCATCAGCCTCGGCTACAC |
| CmFPstI | AAAACTGCAGGTGACGGAAGATCACTTCGCA |
| CmRPstI | AAAACTGCAGGCGTTTAAGGTCAACAATAACTGC |
| Constructing pME6010 hmqL | |
| hmqL-F | CCGAGATCTACCCAATTCATAGACCAGCGTTGC |
| hmqL-R | CCGGGTACCTCATGATGCGTACCTCCGTCGATT |
| Constructing the hmqL::dhfR mutant by natural transformation | |
| hmqL-Tn-for2 | CGTCATGCCCAATGTGCGCTTG |
| hmqL-Tn-rev2 | GTTGGTTGACGACTGCGCGAAC |