FIG 2.
Adenylation domain-mediated lipoinitiation of bogorol peptides. (A) Production of bogorols by the wild-type (WT) and ΔbogJ mutant strains of B. laterosporus LMG15441. Strains were grown in MEM broth for 48 h and extracted with a C18 column filled with silica gel. The extracted products were subsequently subjected to HPLC. (B) Phylogenetic tree of A domains from the bogorol gene cluster harbored by B. laterosporus MG64 and reference A domains that incorporate hydroxy acid. Auriporcine was retrieved from B. laterosporus PE36, while cereulide and valinomycin were retrieved from the miBIG database. The A domains activating alpha-hydroxy acids are indicated in red. (C) Usual pattern of fatty acid incorporation in NRPs. CoA, coenzyme A; FACL, fatty acid coenzyme ligase. (D) Proposed pattern of bogorols incorporating fatty acid.