TABLE 3.
Primers used in this study
| Primer | Sequencea | Purpose |
|---|---|---|
| bogJ-sgRNA-F | TACGATTAGTAGAAAGTGTTTACG | Knockout of bogJ |
| bogJ-sgRNA-R | AAACCGTAAACACTTTCTACTAAT | Knockout of bogJ |
| bogJ-up-SfiI-F | AAGGCCAACGAGGCCGTAGCTCCGCTTCACAGTAG | Knockout of bogJ |
| bogJ-up-R | GGCGTTCTTCCTTGTCTCCTCCTGCTCTATG | Knockout of bogJ |
| bogJ-down-F | GGAGGAGACAAGGAAGAACGCCCTATTTCTG | Knockout of bogJ |
| bogJ-down-SfiI-R | AAGGCCTTATTGGCCGCTTGTAACGCTTGCAATG | Knockout of bogJ |
| bogI-sgRNA-F | TACGGAAGCCGTCTCAGTTAGACG | Knockout of bogI |
| bogI-sgRNA-R | AAACCGTCTAACTGAGACGGCTTC | Knockout of bogI |
| bogI-up-SfiI-F | AAGGCCAACGAGGCCTATGTAGCGGCTGTAAAGGG | Knockout of bogI |
| bogI-up-R | AGTAGGTGGCCACGGGAGCACTTTAACTCAG | Knockout of bogI |
| bogI-down-F | AGTGCTCCCGTGGCCACCTACTAAGGAAGAG | Knockout of bogI |
| bogI-down-SfiI-R | AAGGCCTTATTGGCCTAACCCGATCTTGAGGCTTG | Knockout of bogI |
| pJOE8999-check-F | CGTGTGATGCGAATTCTTGAC | Confirmation of cloning |
| pJOE8999-check-R | GATGCCACTCTTATCCATCAATCC | Confirmation of cloning |
a
The restriction sites are indicated in boldface type, while base-pairing regions in the sgRNA primers are indicated by underlining.