Phylogenic tree based on MLST analysis. The minimum spanning tree was generated using GrapeTree from the sequence types of all 896 C. coli isolates, based on 7 MLST genes (aspA, glnA, gltA, glyA, pgm, tkt, and uncA) extracted using the PubMLST platform. Orange represents isolates isolated from chickens, green from cattle, and magenta from pigs. Red is for clinical isolates. Circle sizes are proportional to the number of isolates, and the scale bar represents a genetic distance of 1.