TABLE 2.

PCR primers used in the study to generate the A1 domain of PWD MEFA and PWD MEFA genes

Primer	Sequence (5′→3′)	Amplified region
PWD-MEFA-F	CGGGCTAGCATGAAAAATATAACTTTC	Upstream of A1 domain of PWD MEFA, with NheI site
PWD-MEFA-R	TTACGGCCGCTAGTTTTCCATACTGAT	Downstream of LTB gene, with EagI site
PWD-MEFA-A1-L	TCATTACAAGTATCACCTGTAATTGTTCTTGAATAATTTTCACAC	Overlapping LTA1 domain with A2 domain
PWD-MEFA-A2-R	AAATTATTCAAGAACAATTACAGGTGATACTTGTAATGAGGAGAC	Overlapping LTA1 domain with A2 domain
PWD-MEFA-A1-F	CGGGCTAGCCCGATGAAAAACATCACCTTTATC	Upstream of A1 domain of PWD MEFA, with NheI site
PWD-MEFA-A-R	TTACGGCCGGAAGATGGTACGGCTGTAGTTCTC	Downstream of A1 domain of PWD MEFA, with EagI site