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. 2020 Nov 24;86(24):e01915-20. doi: 10.1128/AEM.01915-20

TABLE 3.

Strains and plasmids used in this study

Strain or plasmid Relevant characteristics Reference
Strains
 C. glabrata ATCC 2001 51
 C. glabrata HTUΔ his3Δ trp1Δ ura3Δ 51
    CgMed2Δ his3Δ trp1Δ ura3Δ CgMED2::CgHIS3 This study
    CgMed2Δ/CgMED2 his3Δ trp1Δ ura3Δ med2Δ CgMED2::CgHIS3 pY26-PGPD/CgMED2 This study
    CgYap6Δ his3Δ trp1Δ ura3Δ CgYAP6::CgTRP1 This study
    CgHap5Δ his3Δ trp1Δ ura3Δ CgHAP5::CgHIS3 This study
    CgCom2Δ his3Δ trp1Δ ura3Δ CgCOM2::CgHIS3 This study
    CgAft1Δ his3Δ trp1Δ ura3Δ CgAFT1::CgHIS3 This study
    CgSut1Δ his3Δ trp1Δ ura3Δ CgSUT1::CgHIS3 This study
    CgYap5Δ his3Δ trp1Δ ura3Δ CgYAP5::CgHIS3 This study
    CgYak1Δ his3Δ trp1Δ ura3Δ CgYAK1::CgHIS3 This study
    CgMed2ΔCgyap6Δ his3Δ trp1Δ ura3Δ CgYAP6::CgTRP1 CgMED2::CgHIS3 This study
    CgYap6Δ/CgYAP6 his3Δ trp1Δ ura3Δ yap6Δ CgYAP6::CgTRP1 pY26-PGPD/CgYAP6 This study
    CgHap5Δ/CgHAP5 his3Δ trp1Δ ura3Δ hap5Δ CgHAP5::CgHIS3 pY26-PGPD/CgHAP5 This study
    CgCom2Δ/CgCOM2 his3Δ trp1Δ ura3Δ com2Δ CgCOM2::CgHIS3 pY26-PGPD/CgCOM2 This study
    CgAft1Δ/CgAFT1 his3Δ trp1Δ ura3Δ aft1Δ CgAFT1::CgHIS3 pY26-PGPD/CgAFT1 This study
    CgSut1Δ/CgSUT1 his3Δ trp1Δ ura3Δ sut1Δ CgSUT1::CgHIS3 pY26-PGPD/CgSUT1 This study
    CgYap5Δ/CgYAP5 his3Δ trp1Δ ura3Δ yap5Δ CgYAP5::CgHIS3 pY26-PGPD/CgYAP5 This study
    AH109 trp1Δ leu2Δ ura3Δ his3Δ gal4Δ gal80Δ LYS2::GAL1UAS-GAL1TATA-HIS3GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MEL1TATA-LacZMEL1 This study
    CgMed2Δ/CgMED2-Myc his3Δ trp1Δ ura3Δ CgMED2::CgHIS3 pY26-PGPD/CgMed2-Myc This study
    CgMed2Δ/CgYap6Δ/CgMed2-Myc/CgYap6-His his3Δ trp1Δ ura3Δ CgYAP6::CgTRP1 CgMED2::CgHIS3 pY26-PGPD/CgMed2-Myc pY26-PTEF/CgYap6-His This study
Plasmids
    pY26 2μ Amp URA3 PGPD PTEF This study
    pGBKT7 Kan TRP1 GAL4 DNA-binding domain fusion This study
    pGADT7 Amp LEU2 GAL4 DNA-binding domain fusion This study