TABLE 4.
Primers used in this study
| Category and name | Sequence (5′→3′)a |
|---|---|
| Deletion | |
| L-CgMED2-F1 | TACAAATTAGCTATTATTACCAA |
| L-CgMED2-F2 | CTTAACAAACGCCATTACTTTCAGACGGGCAGTTTAT |
| CgHIS3(CgMed2)-F1 | GCCCGTCTGAAAGTAATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgMed2)-F2 | AATAGGAAGACCAAGCTATGCTAGGACACCCTTAG |
| R-CgMED2-F1 | GGTGTCCTAGCATAGCTTGGTCTTCCTATTGTTAACTATT |
| R-CgMED2-F2 | GGATAAATTTTTTGATAGTTTAGTA |
| L-CgYAP6-F1 | TTTATCAGGAACAAGCTGTTAC |
| L-CgYAP6-F2 | AGTAACGAATCAAATGACATTATATCTCCGGAAATATAGT |
| CgTRP1(CgYAP6)-F1 | ACTATATTTCCGGAGATATAATGTCATTTGATTCGTTACT |
| CgTRP1(CgYAP6)-F2 | AGTGCATTCGGAGTTCAGAGTCATTGTTTCTTTGCATTTTGTACA |
| R-CgYAP6-F1 | AAAATGCAAAGAAACAATGACTCTGAACTCCGAATGCACT |
| R-CgYAP6-F2 | AAAAGCATCTGTCACAGAAAAG |
| L-CgHAP5-F1 | TTTGTAGTGCCGCTTTCCC |
| L-CgYAP5-F2 | ACCCTCTTAACAAACGCCATTACCCACTTCTAATATATAAACCTG |
| CgHIS3(CgHap5)-F1 | TTATATATTAGAAGTGGGTAATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgHap5)-F2 | TCAGGATTACATACTACATACTATGCTAGGACACCCTTAG |
| R-CgHAP5-F1 | CTAAGGGTGTCCTAGCATAGTATGTAGTATGTAATCCTGAATAAG |
| R-CgHAP5-F2 | ACAACTTTTTCTTGGTATAATTTAT |
| L-CgCOM2-F1 | CTTTCCTTTAATGCTCTCGA |
| L-CgCOM2-F2 | ACCCTCTTAACAAACGCCATTACTCCGTAGTGTATAATTGT |
| CgHIS3(CgCom2)-F1 | CAATTATACACTACGGAGTAATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgCom2)-F2 | TTGCATTCAAGCAAATACCACTATGCTAGGACACCCTTAG |
| R-CgCOM2-F1 | CTAAGGGTGTCCTAGCATAGTGGTATTTGCTTGAATGCAAAA |
| R-CgCOM2-F2 | CCTCATTAAGTGATGACGAACTACA |
| L-CgAFT1-F1 | TATAAGGAAGGAAAAGTTAAGACGT |
| L-CgAFT1-F2 | ACCCTCTTAACAAACGCCATAGCATTTCAATATTTCAATAAGAAA |
| CgHIS3(CgAft1)-F1 | TATTGAAATATTGAAATGCTATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgAft1)-F2 | CATCTCATATCAATCAATATCTATGCTAGGACACCCTTAG |
| R-CgAFT1-F1 | CTAAGGGTGTCCTAGCATAGATATTGATTGATATGAGATGTATTG |
| R-CgAFT1-F2 | TCTGTACCATCTTTTATATGCAATC |
| L-CgSUT1-F1 | AAAAAGAAAAAGCTATAGCAAGGAG |
| L-CgSUT1-F2 | ACCCTCTTAACAAACGCCATTTCGTTAATTTTTAGTTTATGCTTT |
| CgHIS3(CgSut1)-F1 | ATAAACTAAAAATTAACGAAATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgSut1)-F2 | CTTTAGTTATCGATGGTAAACTATGCTAGGACACCCTTAG |
| R-CgSUT1-F1 | CTAAGGGTGTCCTAGCATAGTTTACCATCGATAACTAAAGCCTTA |
| R-CgSUT1-F2 | GCCTTTTGTGTTTAAGTTGC |
| L-CgYAP5-F1 | ATGCGAACCCTTCGCC |
| L-CgYAP5-F2 | ACCCTCTTAACAAACGCCATCGGGTATCCACCGCTAG |
| CgHIS3(CgYap5)-F1 | GGCCTAGCGGTGGATACCCGATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgYap5)-F2 | AAGCTATTCTACAGCGATAACTATGCTAGGACACCCTTAG |
| R-CgYAP5-F1 | CTAAGGGTGTCCTAGCATAGTTATCGCTGTAGAATAGCTTAATAT |
| R-CgYAP5-F2 | AAAACTGTGACTTTCTGCCT |
| L-CgYAK1-F1 | ACTATATCAGCAGCTACGAGCC |
| L-CgYAK1-F2 | CCCTCTTAACAAACGCCATCGAAGATAATCTGCCCCTTCA |
| CgHIS3(CgYak1)-F1 | GAAGGGGCAGATTATCTTCGATGGCGTTTGTTAAGAGGGT |
| CgHIS3(CgYak1)-F2 | TTAATGAAATGGAGATAAAACTATGCTAGGACACCCTTAG |
| R-CgYAK1-F1 | CTAAGGGTGTCCTAGCATAGTTTTATCTCCATTTCATTAAATCTT |
| R-CgYAK1-F2 | TGGGGACAACTCACTGGAT |
| Overexpression | |
| CgMED2-F1(pY26) | GATTCTAGAACTAGTGGATCCATGAGTTACAAGAACAGGCTTACGG |
| CgMED2-F2(pY26) | GTCGACGGTATCGATAAGCTTTTAGATATTAAAGCCATTTAGGTCTAGGTC |
| CgYAP6-F1(pY26) | GATTCTAGAACTAGTGGATCCATGGGACAAGTTAACATGCGACC |
| CgYAP6-F2(pY26) | GTCGACGGTATCGATAAGCTTCTAGGACTTCTCGCCAGCAATT |
| CgHAP5-F1(pY26) | GATTCTAGAACTAGTGGATCCATGGAGAAGATGGAAAAGACGTATG |
| CgHAP5-F2(pY26) | GTCGACGGTATCGATAAGCTTTTACGAAGAGTTGTTTTGCGCTC |
| CgCOM2-F1(pY26) | GATTCTAGAACTAGTGGATCCATGACGGACACATTTCAGCTGG |
| CgCOM2-F2(pY26) | GTCGACGGTATCGATAAGCTTTCAATGGCTATGTGTTTTTATGTGTT |
| CgAFT1-F1(pY26) | GATTCTAGAACTAGTGGATCCATGGATTCCAACCAACTAATACACTT |
| CgAFT1-F2(pY26) | GTCGACGGTATCGATAAGCTTTCACATTATGTGATCTTCTTCTAATTTAACA |
| CgSUT1-F1(pY26) | GATTCTAGAACTAGTGGATCCATGGCTACAAGTATAACTGTTTTGAATAGA |
| CgSUT1-F2(pY26) | GTCGACGGTATCGATAAGCTTTTAGAATCCTGCCTTCTTGTATTCC |
| CgYAP5-F1(pY26) | GATTCTAGAACTAGTGGATCCATGCTGACTGCTCTGGGATCA |
| CgYAP5-F2(pY26) | GTCGACGGTATCGATAAGCTTCTATGTTCTTTGTCTTTTCGGGG |
| Yeast two-hybrid assay | |
| BD-CgMED2-F1 | TTGACTGTATCGCCGGAATTCATGAGTTACAAGAACAGGCTTACGG |
| BD-CgMED2-F2 | CTATAGGGCTCTAGAGTCGACTTAGATATTAAAGCCATTTAGGTCTAGGTC |
| AD-CgYAP6-F1 | AAAGAGATCGAATTAGGATCCATGGGACAAGTTAACATGCG |
| AD-CgYAP6-F2 | AAGCATTAGAGAATTGAATTCCTAGGACTTCTCGCCAGCA |
| Coimmunoprecipitation | |
| pY26/PGPD-CgMED2-F1 | ATTCTAGAACTAGTGGATCCATGAGTTACAAGAACAGGCT |
| pY26/PGPD-CgMED2-F1 | TCGACGGTATCGATAAGCTTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCGATATTAAAGCCATTTAGGTCTAGG |
| pY26/PTEF-CgYAP6-F1 | GAATTGTTAATTAAAGATCTCTAGTGGTGGTGGTGGTGGTGGGACTTCTCGCCAGCAATTG |
| pY26/PTEF-CgYAP6-F2 | CAGTTAACTCCGGACCGCGGATGGGACAAGTTAACATGCG |
| RT-PCR | |
| YAP5-F1 | GGAGGATTCCAAATGCTA |
| YAP5-F2 | GTTGCTCAAGAATTCGTC |
| HAP5-F1 | ACGAGATAGAGTCTACGA |
| HAP5-F2 | GCGAATATTATAGGTGCC |
| COM2-F1 | GAGGAACTACTTCAATAACATA |
| COM2-F2 | CGTCATTAAGAGTCATTACA |
| YAP6-F1 | GCATCAGTACACCACTAG |
| YAP6-F2 | GACCATTTTCCGAAGAGA |
| AFT1-F1 | GCCAGATCGACTAATAAC |
| AFT1-F2 | TGGTGACATGTATATTGAC |
| SUT1-F1 | CGTTGAGTAGTGTACCAA |
| SUT1-F2 | AGCCTTCTTCAGTTCTAG |
| SCT1-F1 | AGTGCATTGGTATTTTCC |
| SCT1-F2 | TGAGGATGGAAGTAATTCA |
| CDS1-F1 | ACCTGACTTGTGATTTGA |
| CDS1-F2 | TTGGCTTAACGGTAATGA |
| PAH1-F1 | CTCTGATGCTATTGATAAAGG |
| PAH1-F2 | CACAGTACGATAGGACAG |
| ALE1-F1 | GCTGAAGAGATTGCCTAA |
| ALE1-F2 | AGTCGCATAGGTGAATAG |
| PSD1-F1 | CCGAAGAGACTAACCTATA |
| PSD1-F2 | CCAGTTTATTGGTGAATGA |
| CHO1-F1 | AAGGGCAAGTCTAAGTTC |
| CHO1-F2 | GCAAGAAGAAGATGAAGATG |
| OPI3-F1 | GCTCCATACTTCTACTCTG |
| OPI3-F2 | CAGCAATCTTGGTTAGGA |
a
Underlining indicates sequences of regions flanking a target gene or a restriction site.