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. Author manuscript; available in PMC: 2020 Nov 25.
Published in final edited form as: Cell Rep. 2020 Oct 13;33(2):108258. doi: 10.1016/j.celrep.2020.108258

Figure 1. Loss of PD-L1 Leads to a Reduction in the Number of Dendritic Cells (DCs) in the Draining Lymph Node (dLN) following Poly(I:C).

Figure 1.

(A) Flow cytometry gating strategy for conventional DC subsets and myeloid cells in the LN from WT and Pdl1−/− mice. PD-L1 expression shown is from naive mice and mice 24 h following poly(I:C) injection.

(B) Quantification of PD-L1 expression on WT conventional dendritic cell (cDC) 1 and cDC2 at baseline and 24 h following poly(I:C), as well as Pdl1−/− DCs.

(C) Number of cDC1 and cDC2 in the dLN of WT and Pdl1−/− before and 24 h after intradermal poly(I:C) injection (10 μg/mouse). Data are shown from axillary and brachial LNs combined.

(D) Number of CD11b+ CD11c− cells in the dLN of WT and Pdl1−/− mice. Data are shown from axillary and brachial LNs combined.

(E) Number and percentage of CD11c-high cells in the skin from the site of injection of WT and Pdl1−/− mice based on weight.

For (B–E), n = 3 mice per group. Data are shown from one experiment that was repeated four times with similar results. Statistical analysis was done using a one-way ANOVA. *p < 0.05; **p < 0.01; ****p < 0.0001; n.s., p > 0.05. Error bars indicate standard error of the mean. See also Figure S1.