KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD11c clone N148 APC-Cy7 | Biolegend | Cat#117324; RRID: AB_830649 |
| Anti-mouse CD11b clone M1/70 PE-Cy7 | Biolegend | Cat#101216; RRID: AB_312799 |
| Anti-mouse XCR1 clone ZET PerCP-Cy5.5 | Biolegend | Cat#148208; RRID: AB_2564364 |
| Anti-mouse I-A/I-E clone M5/114.15.2 APC | Biolegend | Cat#107614; RRID: AB_313329 |
| Anti-mouse CD40 clone 3/32 PerCP-Cy5.5 | Biolegend | Cat#124624; RRID: AB_2561474 |
| Anti-mouse CD80 clone 16-10A1 APC | Biolegend | Cat#104714; RRID: AB_313135 |
| Anti-mouse CD86 clone GL-1 PE | Biolegend | Cat#105008; RRID: AB_313151 |
| Anti-mouse B220 clone RA3-6B2 BV510 | Biolegend | Cat#103248; RRID: AB_2650679 |
| Anti-mouse PD-L1 clone 10F.9G2 BV421 | Biolegend | Cat#124315; RRID: AB_10897097 |
| Anti-mouse CCR7 clone 4B12 BV421 | Biolegend | Cat#120120; RRID: AB_2561446 |
| Anti-mouse CD8 clone 53-6.7 APCcy7 | Biolegend | Cat#100714; RRID: AB_312753 |
| Anti-mouse CD44 clone IM7 PerCPcy5.5 | Biolegend | Cat#103032; RRID: AB_2076204 |
| Anti-mouse CD3 clone 17-A2 Pacific Blue | Biolegend | Cat#100214; RRID: AB_493645 |
| H2Kb SIINFEKL tetramer PE | NIH Tetramer Core Facility | https://tetramer.yerkes.emory.edu/reagents/class-I-mhc |
| Anti-mouse CD40 | BioXcell | Cat#BE0016-2; RRID: AB_1107647 |
| Anti-phosphoAKT | Cell Signaling | Cat#9271S; RRID: AB_329825 |
| Anti-phosphoAKT | Cell Signaling | Cat#4060S; RRID: AB_2315049 |
| Anti-ERK1/2-Total | Biolegend | Cat# 686902; RRID: AB_2629535 |
| Anti-phosphoERK1/2 | Cell Signaling | Cat#9101S; RRID: AB_331646 |
| Anti-GAPDH-HRP | Invitrogen | Cat#MA5-15738-HRP; RRID: AB_2537659 |
| Anti-p38 MAPK | Cell Signaling | Cat#8690S; RRID: AB_10999090 |
| Anti-phosphop38 MAPK | Cell Signaling | Cat#4511S; RRID: AB_2139682 |
| Anti-STAT3 | Cell Signaling | Cat#4904S; RRID: AB_331269 |
| Anti-phosphoStat3 (Tyr705) (D3A7) | Cell Signaling | Cat#9145S; RRID: AB_2491009 |
| Anti-Rabbit IgG H&L (HRP) | Abcam | ab7090; RRID: AB_955417 |
| Anti-Rat IgG H&L (HRP) | Abcam | ab97057; RRID: AB_10680316 |
| Bacterial and Virus Strains | ||
| Listeria monocytogenes-expressing ovalbumin | Ross Kedl | N/A |
| Biological Samples | ||
| Murine lymph nodes | This paper | N/A |
| Murine skin | This paper | N/A |
| Murine bone marrow | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Fluorescein isothiocyanate isomer 1 | Sigma-Aldrich | Cat#F7250 |
| polyI:C | Invivogen | Cat#Vac-PIC |
| Recombinant Murine Exodus-2 (CCL21) | Peprotech | Cat#250-13 |
| Recombinant Murine MIP-3β (CCL19) | Peprotech | Cat#250-27B |
| Recombinant Murine SDF-1β (CXCL12) | Peprotech | Cat#250-20B |
| Sphingosine-1-phosphate | R&D Systems | Cat#1370/1 |
| Ghost Dye Red 780 | Tonbo Biosciences | Cat#13-0865 |
| Violet proliferation dye | BD Biosciences | Cat#562158 |
| Critical Commercial Assays | ||
| IP-One Gq kit | Cis-Bio | Cat#62IPAPEB |
| MojoSort Mouse CD8 T Cell Isolation Kit | Biolegend | Cat#480008 |
| F-actin Visualization Biochem Kit (fluorescence format) | Cytoskeleton Inc. | Cat#BK005 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Pdl1−/−: CD274 KO (C57BL/6) | Mayo Clinic | N/A |
| Mouse: OT1: C57BL/6-Tg(TcraTcrb) 1100Mjb/J | Jackson Labs | JAX: 003831 |
| Mouse: WT: C57BL/6 | Charles River Labs | C57BL/6 (B6) Mouse Inbred 027 |
| Mouse: Pdl1CyMt: CD274(TSS277-279AAA) | Regional Mouse Genomics Core at National Jewish Health, This paper | N/A |
| Oligonucleotides | ||
| Mm_Rn18s_3_SG QuantiTect Primer Assay | QIAGEN | Cat#QT02448075 |
| Mm_Ccr7_1_SG QuantiTect Primer Assay | QIAGEN | Cat#QT00240975 |
| Guide RNA: ACAAGCTCAAAAAACCGAAA TGG | Synthego | N/A |
| Recombinant DNA | ||
| ssDNA HDR template: TTAAGATTGATTTCTTCTTCTTTAGTGAGAATGCTAGATGTGGAGAAATGTGGCGTTGAAGATgctgcagccAAAAACCGAAATGGTAAGTGTGAGTAACGAGGGAGGGGCAAGCCGAGGGAATGAGTGGGACA. | IDT | N/A |
| Software and Algorithms | ||
| Prism Version 8 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| CRISPOR | Jean-Paul Concordet and Macimilian Haeussler | http://crispor.tefor.net/ |
| GPP sgRNA Designer: CRISPRko | Broad Institute | https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design |
| FlowJo | Becton, Dickinson and Company | https://www.flowjo.com/ |