Figure 2.

Inhibition of the IPN-vHipp pathway dramatically increases active escape behaviors and sucrose consumption. A, Mice were injected with the retrograde AAV-cre bilaterally in the vHipp and the cre-dependent AAV-hM4Di DREADD (M4) or AAV control vector (CV) in the IPN. Mice expressing hM4Di in the IPN-vHipp exhibited a decrease in (B) time immobile and (C) number of immobile bouts following CNO injections relative to vehicle (VEH) injections. No differences were found in the control vector mice (CV) injected with CNO or vehicle. D, CNO-mediated inhibition of hM4Di-expressing IPN-vHipp neurons also increased sucrose consumption. No differences were found in the control mice injected with CNO or vehicle; *p < 0.05.