Table 1. Cryo-EM data acquisition, reconstruction, and model refinement statistics.
| hSARM1 construct and pretreatment |
hSARM1 GraFix-ed |
hSARM1 +5 mM NAD+ |
hSARM1 no treatment |
SAM1–2 |
|---|---|---|---|---|
| Electron microscope | Titan Krios | Titan Krios | F30 Polara | F30 Polara |
| Cryo-EM acquisition and processing | ||||
| EMDB accession # | 11187 | 11834 | 11190 | 11191 |
| Magnification | 165,000x | 165,000x | 200,000 | 200,000 |
| Voltage (kV) | 300 | 300 | 300 | 300 |
| Total electron exposure (e−/Å2) | 50 | 40 | 80 | 80 |
| Defocus range (µM) | −0.8 to −2.8 | −0.8 to −2.8 | −1.0 to −2.5 | −1.0 to −2.5 |
| Pixel size (Å) | 0.827 | 0.827 | 1.1 | 1.1 |
| Symmetry imposed | C8 | C8 | C8 | C8 |
| Initial particles | 658,575 | 335,526 | 26,496 | 416,980 |
| Final particles | 147,232 | 159,340 | 5410 | 43,868 |
| Resolution (masked FSC = 0.143 Å) | 2.88 | 2.7 | 7.7 | 3.77 |
| Model refinement | ||||
| PDB ID | 6ZFX | 7ANW | 6ZG0 | 6ZG1 |
| Model resolution (FSC = 0.50/0.143 Å) | 3.6/2.9 | 2.9/2.7 | ||
| Model refinement resolution | 2.88 | 2.70 | ||
| Non-hydrogen atoms | 39,856 | 40,560 | ||
| Residues | 5080 | 5176 | ||
| RMS deviations | ||||
| Bond length (Å) | 0.008 | 0.009 | ||
| Bond angle (°) | 1.84 | 1.84 | ||
| Ramachandran plot | ||||
| Favored (%) | 89.05 | 91.68 | ||
| Allowed (%) | 10.95 | 8.32 | ||
| Disallowed (%) | 0 | 0 | ||
| Rotamer outliers (%) | 5.73 | 6.64 | ||
| Validation | ||||
| MolProbity score | 2.68 | 2.49 | ||
| Clash score | 10.08 | 6.66 | ||