Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Rat monoclonal anti-mouse CD34 (RAM34) FITC |
ThermoFisher Scientific | Cat# 11-0341-82, RRID:AB_465021 |
FC (5 ug/mL) |
| Antibody | Rat monoclonal anti-mouse Lineage cocktail A700 (anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119) |
Bio-Legend | Cat# 133313, RRID:AB_2715571 |
FC (5 uL/mouse) |
| Antibody | Rat monoclonal anti-mouse cKIT (2B8) APC-eFluor780 |
ThermoFisher Scientific | Cat# 47-1171-82, RRID:AB_1272177 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse Sca1 (D7) PE-Cy7 |
Bio-Legend | Cat# 108114, RRID:AB_493596 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse CD150 (TC15-12F12.2) APC |
Bio-Legend | Cat# 115910, RRID:AB_493460 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse Flt3 (A2F10) PerCP-eFluor710 |
ThermoFisher Scientific | Cat# 46-1351-82, RRID:AB_10733393 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse CD16/32 (2.4G2) BUV395 |
BD Biosciences | Cat# 740217, RRID:AB_2739965 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse IL7Ra (A7R34) APC |
Bio-Legend | Cat# 135012, RRID:AB_1937216 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse CD150 (TC15-12F12.2) BV421 |
Bio-Legend | Cat# 115925, RRID:AB_10896787 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse IL7Ra (SB/199) BV711 |
BD Biosciences | Cat# 565490, RRID:AB_2732059 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse ESAM (1G8) APC |
Bio-Legend | Cat# 136207, RRID:AB_2101658 |
FC (2 ug/mL) |
| Antibody | Rat monoclonal anti-mouse CD16/32 (93) PE |
Bio-Legend | Cat# 101307, RRID:AB_312806 |
FC (2 ug/mL) |
| Antibody | Rabbit monoclonal anti-mouse Pfkl (EPR11904) |
Abcam | Cat# ab181064, RRID | IF (1:100) |
| Antibody | Cy3 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG |
Jackson ImmunoResearch | Cat# 711-166-152, RRID:AB_2313568 |
IF (1:500) |
| Commercial assay or kit |
iST NHS 96x | PreOmics | iSTNHS96x | |
| Commercial assay or kit |
Pierce Quantitative Colorimetric Peptide Assay |
ThermoFisher Scientific | 23275 | |
| Commercial assay or kit |
RNAeasy minelute CleanUp Kit | QIAGEN | 74204 | |
| Commercial assay or kit |
NEBNext Ultra DNA Library Prep Kit for Illumina |
New England BioLabs | E7103 | |
| Other | TRIzol | Invitrogen | 15596018 | |
| Other | RQ1 RNase free DNase | Promega | M6101 | |
| Other | Agencourt Ampure XP | Beckman Coulter | A63881 |