Fig. 2.

Demonstration of N/C calculation process with (a)–(d) an IDC and (e)–(h) a normal sample. (a) and (e) A $4\times 4{\mathrm{mm}}^2$ area from the fluorescence images of an IDC and a normal sample, respectively. (b) and (f) The binary image after nuclei segmentation from the same region as in (a and e). (c) and (g) The calculated N/C map of small patches ($250\times 250{\mu \mathrm{m}}^2$), with color bar on right signifying N/C values. (d) and (h) N/C of large patches ($2\times 2{\mathrm{mm}}^2$) obtained by averaging over $8\times 8$ small patches from (c) and (g). As seen in color bars on right of N/C maps, low N/C ratios are represented by blue shades; higher N/C ratios are depicted by green, yellow, orange and red shades.