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. 2020 Nov 17;2020:5925342. doi: 10.1155/2020/5925342

Figure 3.

Figure 3

HOTAIR positively regulates Cx43 by attenuating suppressive effect of miR-613 on the Cx43 expression. (a) The efficacy evaluation of the expression vector of HOTAIR and HOAIR siRNA after transfection; ∗P < 0.05, against the blank group, HOTAIR siRNA NC group, or HOTAIR NC group. (b) HOTAIR has no direct effect on the miR-613 expression in HL-1 cell. (c) HOTAIR positively regulates the Cx43 expression in HL-1 cell. GAPDH was used as an internal control; ∗P < 0.05, against the blank group, HOTAIR siRNA NC group, or HOTAIR NC group. (d) HOTAIR counteracts the suppressive effect of miR-613 on the Cx43 expression. GAPDH was used as an internal control; ∗P < 0.05, against the control group; #P < 0.05, against the miR-613 group or miR-613 + HOTAIR NC group. (e) miR-613 counteracts the upregulated effect of HOTAIR on the Cx43 expression. GAPDH was used as an internal control; ∗P < 0.05, against the control group; #P < 005, against the HOTAIR group or HOTAIR+miR-613 NC group. miR: microRNA; HOTAIR: HOX transcript antisense RNA; Cx: connexin; GAPDH: glyceraldehyde-3-phosphate dehydrogenase. The error bars mean standard deviation.