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RT-PCR (a) and qRT-PCR (a) to clarify the FL TrkB and TrkB-T1 isoform expression in lung adenocarcinoma cell lines (NCI-H460) grown in adherence (AD) or in spheroid conditions (SP). The total amount of cDNA present in each reaction was normalized by β-actin gene amplification. (a) The RT-PCR display and increased expression of the FL/T1 ratio in spheroid cancer cell. This data was confirmed by qRT-PCR (b). AD, adherent cell lines; SP, spheroid cell lines.
