Table 3: Densitometric analysis of the expression of ENaC subunits in kidney cortex from nphs2^Δipod *plg^+/+and nphs2^Δipod *plg^−/−mice before and after induction of nephrotic syndrome and under treatment with aprotinin.

Signal intensity of the bands (relative units) was normalized for total protein expression of the respective lane.

		uninduced		nephrotic + placebo		nephrotic + aprotinin
	molecular weight, kDa	nphs2Δipod*plg+/+	nphs2Δipod*plg−/−	nphs2Δipod*plg+/+	nphs2Δipod*plg−/−	nphs2Δipod*plg+/+	nphs2Δipod*plg−/−
α-ENaC	87	186 ± 16	252 ± 23	229 ± 40	237 ± 29	223 ± 58	183 ± 75
	26	64 ± 12	90 ± 31	110 ± 11	98 ± 53	69 ± 3	95 ± 21
β-ENaC	85	8 ± 1	10 ± 1	8 ± 0.3	10 ± 1	11 ± 1	9 ± 1
γ-ENaC	84	178 ± 36	183 ± 45	142 ± 28	254 ± 37	284 ± 42	188 ± 23
	72	110 ± 9	163 ± 55	187 ± 24#	141 ± 62	97 ± 13§	133 ± 48

Arithmetic means of expression in relative units ± SEM (n=4 each)

^#significant difference between uninduced and nephrotic mice of the same genotype (t-test),

^§significant difference between placebo and aprotinin-treated nephrotic mice (t-test).