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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: FASEB J. 2020 Jun 11;34(8):10117–10131. doi: 10.1096/fj.202000807R

Figure 3.

Figure 3.

Morphological analyses after 48/80 implantation in MC deficient rats and WT littermates. A, B) Choroidal whole mounts labeled for RPE65 at 6 wk post implantation of 48/80-hydrogel in MC deficient WsRCws/ws (WS/WS) and their WT littermate WsRC+/+ (+/+) rats. The graph shows quantification of percentage RPE degeneration (n = 4, per group). Scale bar, 100 μm. C) Images of three-dimensional volume renderings of macrophage/monocytes (Iba1+; green) and MCs (tryptase+; red) in the choroid. No MCs were detected in WsRCws/ws, while MCs were degranulated in WsRC+/+ WT rats at 6 wk. D) The graph shows comparison of volume and sphericity of Iba+ cells in WsRC+/+ (n = 327) and WsRCws/ws (n = 162). Scale bar, 50 μm. E, F) Representative scotopic electroretinogram (ERG) with the stimuli of 0.01, 0.1 and 1 cd.s/m2 at 6 wk. The graph shows b-wave amplitude with the stimulus of 0.01, 0.1 and 1 cd.s/m2 at baseline (n = 4, per group) and 6 wk post implantation (n = 8, per group). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, 2-tailed, unpaired Student’s t test.