. Author manuscript; available in PMC: 2021 Aug 1.

Published in final edited form as: FASEB J. 2020 Jun 23;34(8):10041–10055. doi: 10.1096/fj.202000215R

Table 4.

Acute hypoxia experiment blood gas and chemistry values.

			AR & NOS Blockade				Interaction	Time	Group
	Baseline	Hypoxia	+	++	+++	++++	P-value	P-value	P-value
Fetal arterial blood
pH
Control	7.285 ± 0.041^†	7.262 ± 0.047	7.247 ± 0.047^†	7.231 ± 0.050^†	7.201 ± 0.064^†	7.194 ± 0.067^†	NS	<0.0001	NS
IGF-1	7.263 ± 0.052^†	7.222 ± 0.054	7.188 ± 0.073^†	7.166 ± 0.081^†	7.144 ± 0.087^†	7.092 ± 0.092^†	NS	<0.0001	NS
CO₂ partial pressure (mmHg)
Control	54 ± 3^†	50 ± 4	49 ± 5	48 ± 5	48 ± 5	47 ± 3^†	NS	<0.0001	0.003
IGF-1	59 ± 4^†	57 ± 7	57 ± 6	56 ± 6	56 ± 6	56 ± 5	NS	<0.0001	0.003
O₂ partial pressure (mmHg)
Control	18.2 ± 1.9^†	10.7 ± 1.1	10.8 ± 1.1	11.4 ± 1.1	11.7 ± 1.3^†	12.5 ± 1.4^†	<0.0001	-	-
IGF-1	15.1 ± 2.8	11.1 ± 1.2	11.7 ± 1.7	12.0 ± 1.6	12.5 ± 2.0	12.2 ± 1.5	<0.0001	-	-
Hemoglobin, total (g dL⁻¹)
Control	10.7 ± 1.8^†	11.7 ± 2.1	11.8 ± 1.9	11.9 ± 2.1	11.9 ± 1.9	12.4 ± 2.2^†	NS	<0.001	NS
IGF-1	10.0 ± 2.0^†	10.7 ± 2.3	11.0 ± 3.0	10.9 ± 2.7	10.6 ± 2.3	10.6 ± 2.5^†	NS	<0.001	NS
O₂ content (mL dL⁻¹)
Control	6.4 ± 1.1^†	2.6 ± 0.4	2.5 ± 0.3	2.8 ± 0.4	2.9 ± 0.6	3.2 ± 0.8	<0.0001	-	-
IGF-1	4.0 ± 1.8	2.1 ± 0.4	2.2 ± 0.5	2.2 ± 0.6	2.3 ± 0.6	2.2 ± 0.6	<0.0001	-	-
Glucose (mM)
Control	0.9 ± 0.3	0.9 ± 0.2	1.0 ± 0.2	1.1 ± 0.3	1.3 ± 0.2^†	1.2 ± 0.3^†	0.098	<0.0001	0.024
IGF-1	0.6 ± 0.2	0.7 ± 0.3	0.7 ± 0.3	0.7 ± 0.3^†	0.7 ± 0.3^†	0.8 ± 0.4^†	0.098	<0.0001	0.024
Lactate (mM)
Control	1.9 ± 0.4^†	4.8 ± 1.6	5.5 ± 1.9^†	6.3 ± 1.9^†	7.4 ± 2.6^†	8.4 ± 2.6^†	NS	<0.0001	0.03
IGF-1	4.0 ± 4.1^†	7.3 ± 3.5	9.0 ± 3.6^†	10.2 ± 4.1^†	11.3 ± 4.1^†	11.9 ± 5.0^†	NS	<0.0001	0.03
Hematocrit (%)
Control	36 ± 6^†	39 ± 7	39 ± 7	40 ± 7	40 ± 7	41 ± 7^†	NS	<0.0001	NS
IGF-1	33 ± 7^†	37 ± 8	37 ± 8	38 ± 8	38 ± 8	38 ± 9^†	NS	<0.0001	NS
Fetal coronary sinus blood
pH
Control	7.253 ± 0.041^†	7.243 ± 0.028				7.152 ± 0.051^†	NS 0.052	<0.0001	NS 0.072
IGF-1	7.244 ± 0.061^†	7.181 ± 0.042				7.050 ± 0.065^†	NS 0.052	<0.0001	NS 0.072
CO₂ partial pressure (mmHg)
Control	62 ± 3	54 ± 5				55 ± 2	NS	0.027	0.03
IGF-1	63 ± 4	62 ± 7				61 ± 5	NS	0.027	0.03
O₂ partial pressure (mmHg)
Control	12.4 ± 0.9^†	6.8 ± 1.5				6.1 ± 1.2	NS	<0.0001	NS
IGF-1	10.4 ± 2.2^†	6.3 ± 0.6				6.1 ± 1.1	NS	<0.0001	NS
O₂ content (mL dL⁻¹)
Control	3.2 ± 0.4^†	1.2 ± 0.2				0.9 ± 0.2	0.042	-	-
IGF-1	2.0 ± 0.9 ^*^†	0.8 ± 0.1 ^*				0.7 ± 0.1^†	0.042	-	-
Glucose (mM)
Control	0.58 ± 0.08	0.72 ± 0.16				1.08 ± 0.10	0.029	-	-
IGF-1	0.57 ± 0.27	0.65 ± 0.31				0.72 ± 0.34^†	0.029	-	-
Lactate (mM)
Control	1.52 ± 0.64^†	4.98 ± 1.72				9.38 ± 2.15^†	NS	<0.0001	NS
IGF-1	4.07 ± 4.49^†	8.02 ± 3.49				12.60 ± 4.24^†	NS	<0.0001	NS
Maternal arterial blood
O₂ partial pressure (mmHg)
Control	107.6 ± 10.9^†	40.3 ± 6.0	42.7 ± 4.4	39.3 ± 11.4	42.5 ± 8.2	41.2 ± 12.4	NS	<0.0001	NS
IGF-1	102.7 ± 7.3^†	42.0 ± 7.5	46.3 ± 5.5	40.7 ± 9.0	47.0 ± 8.0	38.8 ± 7.4	NS	<0.0001	NS
O₂ saturation (%)
Control	100 ± 1^†	58 ± 14	64 ± 11	55 ± 22	63 ± 12	57 ± 24	NS	<0.0001	NS
IGF-1	98 ± 3^†	60 ± 16	69 ± 13	58 ± 17	69 ± 11	56 ± 15	NS	<0.0001	NS

Arterial and maternal values Control n=10, except last dose n=9; IGF-1 n=7, except last dose n=6. Coronary sinus values Control n=6, except last dose n=4; IGF-1 n=6, except last dose n=5. Not significant (NS). Arterial values analyzed by 2-way repeated measures ANOVA. Coronary sinus values analyzed by mixed-effects analysis. If justified, further analysis was by the Holm-Šidák test.

Different from same-timepoint Control

^†

same-group Hypoxia timepoint. Data shown as mean ± SD.