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. Author manuscript; available in PMC: 2020 Dec 9.
Published in final edited form as: J Bone Miner Res. 2020 Jul 6;35(10):2004–2014. doi: 10.1002/jbmr.4091

Fig 6.

Fig 6

ATF4 rescues amino acid uptake and proliferation in the absence of GCN2. (A–C) Validation of Atf4 and Ddit3 expression in primary BMSC measured by Western blot (A) or qPCR (B, C). Effect of Atf4 expression on amino acid transporter expression (D), radiolabeled amino acid uptake (E), or EdU incorporation (F, G). Scale bar = 25 μm. Each data point represents primary BMSC isolated from an individual mouse for each genotype. n ≥ 3. Gene expression normalized to β-actin mRNA levels. (H) Model for how GCN2-mediated amino acid sensing facilitates amino acid uptake and cell division.