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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: FASEB J. 2020 Jun 18;34(8):10191–10211. doi: 10.1096/fj.202000366R

Figure 2: Osteocytes support myeloid cell proliferation through Gsα-dependent secreted factor(s).

Figure 2:

A) Quantification of myeloid colonies grown in myeloid-supporting medium and treated with conditioned medium (CM) from Ocy454-Gsα control (Gsα cont CM) and Ocy-454GsαKO (GsαKO CM) cells. B) Treatment with CM from one additional control and two additional Ocy-454GsαKO clones using supporting myeloid medium. C) Microscopic images of myeloid cells after CM treatment in non-supporting medium. D) Quantification of the number of myeloid colonies after treatment with CM from Ocy454-Gsα control and Ocy-454GsαKO cells in non-supporting medium. E) Proliferation of myeloid cells after CM treatment compared to non-treatment control. Each experiment was run in duplicate or triplicate samples and repeated at least three times. (One-way ANOVA)