Figure 6 : Osteocytes-enriched bone explants suppress osteoclast differentiation via Gsα-dependent secreted factors.
A) TRAP staining of osteoclasts after treatment with medium alone (No CM) or CM from Dmp1GsαWT and Dmp1GsαKO OEBEs. B) TRAP activity and C) percentages of surface area covered by osteoclasts. Regular medium (No CM, black bars), CM from Dmp1-GsαWT (light grey bars) or Dmp1-GsαKO (dark grey bars) OEBEs (One-way ANOVA). Error bars represent mean ± SEM. Each experiment was repeated at least 3 times.