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. 2020 Nov 25;11:5974. doi: 10.1038/s41467-020-19767-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic of illumination and virus injection of AAV-DIO-eYFP and AAV-DIO-eNpHR-eYFP in the LPBN of VgluT2-ires-Cre mice. b Yellow illumination (589 nm, 1 Hz, 999-ms pulse width, 10 mW) of LPBN^Vglut2 neurons significantly elevates the PWT in response to mechanical stimulation in both Sham-operated and CPN-ligated VgluT2-ires-Cre mice transfected with AAV-DIO-eNpHR virus, but not in mice transfected with DIO-eYFP virus. c Yellow illumination (589 nm, 1 Hz, 999-ms pulse width, 10 mW) of the LPBN significantly increases the latency of the thermal paw-withdrawal response in the Hargreaves test in both Sham-operated and CPN-ligated VgluT2-ires-Cre mice transfected with AAV-DIO-eNpHR virus, but not in mice transfected with DIO-eYFP virus. d Representative tracks of RTPP illustrating yellow (589 nm) light-evoked behavioral preference in CPN-ligated VgluT2-ires-Cre mice injected with AAV-DIO-eYFP (left) or DIO-eNpHR (right) virus in the LPBN. e, f Quantification of RTPP in CPN-ligated mice before (Pre), during (Light), and after (Post) 10-min yellow illumination of the LPBN in VgluT2-ires-Cre mice injected with AAV-DIO-eYFP (e) or AAV-DIO-eNpHR (f) virus. g Representative tracks in the open field test (OFT) before (Pre), during (Light), and after (Post) 5-min yellow laser illumination (589 nm, 1 Hz, 999-ms pulse width, 10 mW) of LPBN^Vglut2 neurons transfected with DIO-eYFP (upper) or DIO-eNpHR (lower) virus in CPN-ligated VgluT2-ires-Cre mice. h–k Quantification of total distance moved (h, j) and ratio of time spent in the periphery and center (i, k) in the OFT in VgluT2-ires-Cre mice transfected with DIO-eYFP or DIO-eNpHR virus in Sham-operated ((h, i) and CPN-ligated (j, k) mice. All data are presented as mean ± s.e.m. and error bars represent s.e.m. *P < 0.05, **P < 0.01, and ****P < 0.0001. See also Supplementary Table 1 for further statistical information. Source data are provided as a Source Data file.