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. 2020 Nov 12;7:593866. doi: 10.3389/fmolb.2020.593866

FIGURE 4.

FIGURE 4

Effect of citrate on the acetylation of histone H4 and ACLY expression in immortalized human hepatic (IHH) cells. (A) Viability of cells treated with citrate at different concentrations (1, 5, 10, and 20 mM) for 24 h. Data were expressed as percentage of the control group and presented as means ± SD of three replicates from three independent experiments. *p < 0.05, **p < 0.01 cells treated with citrate vs untreated control. (B) Representative western blot of Ac-H4 histone and ACLY protein. Densitometric analysis of the immunoreactive bands performed in three independent experiments of (C) Ac-H4 histone and (D) ACLY protein. Protein levels were normalized with β-actin content. The results from three independent experiments are presented as a percentage of protein levels compared with 100% of the control cells. Data are shown as mean ± SD, *p < 0.05, **p < 0.01, cells treated with citrate vs untreated control. (E) Representative western blot of ACLY and Ac-H4 in IHH cells grown in the presence of 10 μM TSA alone and in combination with 1, 5, and 10 mM citrate for 24 h. Densitometric analysis of the immunoreactive bands performed in three independent experiments of (F) Ac-H4 histone and (G) ACLY. Protein levels were normalized with β-actin content. The results from three independent experiments are presented as a percentage of protein levels compared with 100% of the control cells. Data are shown as mean ± SD, *p < 0.05, cells treated with Trichostatin A (TSA) vs control cells or cells treated with TSA in combination with citrate vs TSA alone.