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Elution of aureocin 4181 in a gradient of water and isopropanol (0 to 100%) containing 0.1% trifluoroacetic acid, during purification in the HPLC column Sephasil Peptide C8 (2nd run), monitored at 214 nm. The arrows indicate fractions 17 (a) and 18 (b) that showed antimicrobial activity and that were used to determine the Mr of the peptides. The gray line represents the isopropanol concentration
