Table 1.
The primers used in the study
| Primer name | Sequence (5′-3′) | Description |
|---|---|---|
| qPf | GGGAGGACGAGAAGGAAAGG | qRT-PCR of ZmPP2C26 gene |
| qPr | AACTGCACGATCACGACACT | |
| qGf | TGAATGGCAAGCTCACTGGT | qRT-PCR of ZmGAPDH gene |
| qGr | TGAATGGCAAGCTCACTGGT | |
| PF0 | TCACTTTACCATTTTTATGCGGGA | Forward primer for P2175 amplification |
| PR | CGGGGCTAGGGTTTTTTTTTCTTTC | Reverse primer for P2175 amplification |
| gus-PF0 | acgacggccagtgccaagcttTCACTTTACCATTTTTATGCGGGA | Forward primers for PCR of r promoter::GUS construct |
| gus-PF1 | acgacggccagtgccaagcttGAGCAAATCGACTCCATCCTT | |
| gus-PF2 | acgacggccagtgccaagcttAGCGTCGCGTCTATCCTTCTC | |
| gus-PF3 | acgacggccagtgccaagcttCTACGAAACCGCAAAGTCCAT | |
| gus-PR | tgtgattgtgatgtatctagaCGGGGCTAGGGTTTTTTTTTCTTTC | Reverse primer for PCR of promoter::GUS construct |
| luc- PF0 | ctatagggcgaattgggtacc TCACTTTACCATTTTTATGCGGGA | Forward primers for PCR of promoter::LUC construct |
| luc- PF1 | ctatagggcgaattgggtaccGAGCAAATCGACTCCATCCTT | |
| luc- PF2 | ctatagggcgaattgggtaccAGCGTCGCGTCTATCCTTCTC | |
| luc- PF3 | ctatagggcgaattgggtaccCTACGAAACCGCAAAGTCCAT | |
| luc- PR | caggaattcgatatcaagcttCGGGGCTAGGGTTTTTTTTT | Reverse primer for PCR of promoter::LUC construct |