Table 1.

Qualitative analysis of phage dispersion and persistency upon oral feeding of ETP-1-enriched artemia

Sampling time	Tissues		Oral feeding
		105 PFUmL−1	108 PFUmL−1	1011 PFUmL−1
12 h	Spleen	–	++	++
	Gut	++++++++	++++++++++	+++++++++++
	Kidney	++++++++	+++++++++	+++++++++++
	Liver	++	++++	+++++
3 days	Spleen	–	–	+
	Gut	–	++	++
	Kidney	–	–	++
	Liver	–	–	–
7 days	Spleen	–	–	–
	Gut	–	–	+
	Kidney	–	–	+
	Liver	–	–	–

A “+” sign shows the presence of phages in the tissues. “−” sign indicates absence of phages.

Phages were examined by spotting 5 μL of sample on to the E. tarda host strain in triplicate

If one or more of the triplicate showed phages, the sample was considered as positive

Number of “+” indicates the tenfold value per dissolved whole tissue volume (457.5 μL)

Original stock concentration used to enrich artemia for 3 days feeding were 2.00 × 10⁵, 2.00 × 10⁸, and 2.00 × 10¹¹ PFUmL⁻¹