FIGURE 5.

Split-GFP studies of GvpF and variants of GvpA_mut. (A) Rf-values of the GvpF/GvpA (WT) and the various F/A_mut transformants. The single substitutions in GvpA are indicated below. F/A_mut transformants with relative fluorescence <6 are labeled in blue. The fluorescence was determined in LAU/mm², and the relative fluorescence in relation to the fluorescence of Hfx. volcanii wild type calculated. Two biological and three technical replicates were performed in each case. (B). Sequence of GvpA and summary of the rf-values of the various GvpA mutants. The sequence of GvpA is presented on top and the structural features α1–β1–β2–α2 shaded in grey. The substitutions in GvpA are summarized underneath; each GvpA contains only a single substitution. Rf < 6 is regarded as weak interaction, whereas rf > 11 is similar to GvpA wild type. The Vac phenotype of the respective ΔA+A_mut transformants is indicated by color: red, Vac negative; green, cylinder shaped; yellow, mini gas vesicles; without color, wild type gas vesicles.