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. 2020 Nov 12;11:579521. doi: 10.3389/fmicb.2020.579521

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Copyright © 2020 Lafond, Tauzin, Bruel, Laville, Lombard, Esque, André, Vidal, Pompeo, Quinson, Perrier, Fons, Potocki-Veronese and Giardina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SDS-PAGE analysis of recombinant RgAga (A) and RgSK (B) expressed in Escherichia coli BL21 cells. (A) (1) Samples from cell extracts of E. coli BL21 pOPINE after induction, (2) cell extracts of E. coli BL21 pOPINE:rgaga before induction, (3) cell extracts of E. coli BL21 pOPINE:rgaga after induction (4) purified fraction of RgAga after elution from affinity chromatography and (MM) molecular weight markers. (B) (1) Samples from cell extracts of E. coli BL21 pOPINE:rgsk before induction, (2) cell extracts of E. coli BL21 pOPINE:rgsk after induction, (3) purified fraction of RgSK after elution from affinity chromatography and (MM) molecular weight markers.