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. 2020 Nov 25;11:5975. doi: 10.1038/s41467-020-19783-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Heat maps of the normalized fold changes of the levels of Hsp90 clients (left), and molecular chaperones and co-chaperones (right) identified by whole-cell MS analyses. b Immunoblots of Hsp90 clients, Hsp90 partners and co-chaperones (n = 3 independent experiments). c Immunoblots of Flag-tagged GR (F-GR) and estrogen receptor α (F-ER) overexpressed in HEK293T cells (n = 2 independent experiments). d Immunoblots of the progesterone receptor (PR) overexpressed in HEK293T cells (n = 2 independent experiments). e Transcriptional activities of overexpressed GR (induced by dexamethasone (DEX)), ERα (induced by β-estradiol (E2)), and the PR (induced by progesterone (PROG)), as assayed with specific luciferase reporter genes (n = 2 biologically independent samples); data are represented as fold change relative to those of WT cells (set to 1). f Immunoblot of the endogenous levels of GR (top, n = 2 independent experiments), and its DEX-induced transcriptional activity determined with a transfected luciferase reporter (bottom, n = 2 biologically independent samples). For box plots, data are represented as the median values and edges of the box plots represent the range of the data. Source data are provided as a Source Data file.