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. 2020 Nov 25;11:5979. doi: 10.1038/s41467-020-19447-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Common workflow to build a UC2 application: (a) Starting with a biological question/idea in need of an imaging device drafted in (b) (inverted incubator microscope) and transferred using UC2 components from the CAD library in (c). After printing and assembling it (d) the device will be placed in its working environment (e.g. incubator) (e) ready to acquire long-term image series of e.g. MDCK cells visualized in (i) and Supplementary Video 6. Remote-control is granted using “smart components” (e.g. cellphone, Raspberry Pi) in (h). Reusing components allows the conversion into a cellphone-microscope (f) or light sheet microscope (g) within minutes (see Supplementary Video 5) and Supplementary Notes 7.8). CL: cylindrical lens, TL: tube lens, L: Laser, LA: LED-array, M: Mirror, MO: Microscope Objective, P-CAM: Detector (smartphone or Raspberry Pi), S: sample positioning stage, F: emission filter, Z: focusing stage.