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. 2020 Nov 12;11:594758. doi: 10.3389/fpls.2020.594758

FIGURE 4.

FIGURE 4

Biological activity of SSExt mIFNγ(SP)10 against SINV. HEK293-T cells were either pre-incubated with DMEM (Mock), 50 ng of protein extracts from N. benthamiana healthy leaves as negative control (N), or different concentrations of positive control (PC, commercial IFNγ) or SSExt mIFNγ(SP)10, as indicated in the panel, for 12 h. Subsequently, cells were infected with SINV-eGFP at an M.O.I of 1.0, and analyzed at 24 hpi. (A) eGFP signals as observed by fluorescence microscopy. Scale bar = 100 μm. (B) The accumulation level of eGFP and actin as analyzed by IB analysis. (C) The relative anti-viral activity of SSExt mIFNγ(SP)10. The standard curve of anti-viral effect was initially established by quantifying the intensity of eGFP signals in cells pre-treated with various concentrations of PC-IFNγ (with specific activity of 2X107 IU/mg protein). The relative activity of SSExt mIFNγ(SP)10 was then estimated using the standard curve.