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. 2020 Nov 12;11:589005. doi: 10.3389/fpls.2020.589005

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Copyright © 2020 Huang, Zhou, Shen, Zhou, Wang and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-qPCR assay of the expression level of 10 genes in WT, ST, and OT seeds before and after artificial aging. (A–C,H) Core genes in the interaction network in Figure 9. (C–E) Genes related to energy metabolism. (F,G) Genes corresponding to serine-type endopeptidase inhibitors. (H–J) Stress-related genes. Data represent mean ± SD (n = 3). Significant differences in gene expression levels among the three rice genotypes were determined by Tukey’s test (^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001). The expression level of each gene in unaged WT seeds was defined as 1.