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(A) Metabolites coverage of the targeted metabolomics method. (B) LOQs of the metabolomics method. (C) Performance of pooled plasma QC samples during sequence analysis. (D) Total ion currents (TICs) of plasma samples. (E) PCA and OPLS-DA score plots of 393 plasma samples from 16 subjects. OPLS-DA model was validated by random permutation test with 100 iterations. No overfitting was observed.
