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. 2020 Nov 12;14:580206. doi: 10.3389/fncel.2020.580206

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Copyright © 2020 Liu, Huang, He, Zhuo, Chen, Ge, Duan, Lu and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OM-MSCs ameliorated OGD/R-induced N₂a injury. (A) Schematic representation of the experimental design and the transwell system used for in vitro experiments. (B) Cell viability was determined using the CCK-8 assay, and data are normalized by control cells (the value of control cells was presented as 1.0). (C) Cell necrosis was determined using the LDH assay, and data are expressed as a percentage of the control. (D) Apoptotic cells are represented as the percentage of Annexin-V single-positive plus Annexin-V/PI double-positive cells. (E) Representative plots of FACS by Annexin V–FITC/PI dual staining. All data are displayed as mean ± SEM (n = 3). (^∗∗P < 0.01 vs. control; ^##P < 0.01 vs. OGD/R).