FIGURE 6.

OM-MSCs mitigate OGD/R-induced ROS overproduction and mitochondrial dysfunction through upregulation of UBIAD1. N2a cells were transfected with UBIAD1-specific siRNA (UBIAD1 RNAi) or a non-specific control siRNA. (A,B) The intracellular ROS levels were examined by DCFH-DA in N₂a cells knocked down by UBIAD1. (C–E) The total SOD, GSH-PX, and MDA activities in N₂a were assessed. (F–G) Determination of the mitochondrial membrane potential was carried out using the JC-1 probe. (H) The ATP content in N₂a cells. (I) The cell viability was examined by CCk-8 assay. (J,K) The apoptotic N2a cells knocked down by UBIAD1. All data are displayed as mean ± SEM (n = 3). (^∗P < 0.05, ^∗∗P < 0.01 vs. Con/control RNAi, ^#P < 0.05, ^##P < 0.01 vs. OGD/R/control RNAi, ^&P < 0.05, ^&&P < 0.01 vs. OGD/R + MSCs/control RNAi, ns. no significance).