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. 2020 Oct 20;19:283–293. doi: 10.1016/j.omto.2020.10.008

Figure 2.

Figure 2

Exosomes Secreted by Dox-Pretreated MSCs Can Significantly Enhance BC Resistance to DOX

(A) Transmission electron microscope (TEM) images of exosomes from MSC-conditioned medium and Dox-treated MSC-conditioned medium. Scale bars, 200 nm. (B) Phenotype analysis of protein markers of CD9, CD63, CD81, TSG 101, and calnexin on vesicles by western blot. (C–F) MDA-MB-231 (C and D) and MCF-7 (E and F) cells were treated with MSC-exo and Dt-MSC-exo, and then treated with Dox (1 μM) for 48 h: (C) CCK-8 (C, left; D), annexin V-APC/7-AAD double-staining (C, middle; F), and LDH cytotoxicity (C, right; E) assays detected the viability of the cells, percentage of apoptotic cells, and cell damage. The exosomes were first removed by centrifugation from the supernatant of MSCs and then and the supernatant was added to MDA-MB-231 (D, left) and MCF-7 (F, left) cells: CCK-8 (D) and LDH cytotoxicity (F) assays detected the viability of the cells, vesicular body, and its corresponding conditioned medium on MDA-MB-231 (D, middle, right) and MCF-7 (F, middle, right) cell activity. The data are mean ± SD, averaged from three separate experiments. ∗p < 0.05, ∗∗p < 0.01. n.s., not significant.