Figure 4.

Downregulation of S100A6 in BCs Lessens the Induction of Dox Tolerance by Dt-MSC-exo

MDA-MB-231 cells and MCF-7 cells were transfected with S100A6 siRNAs and negative control siRNA (nc-siRNA). (A and B) The protein (A) and mRNA (B) expression of S100A6 in MDA-MB-231 cells and MCF-7 cells after treatment with Dt-MSC-exo and MSC-exo (B). (C–F) Analysis by CCK8 viability (C and E) and apoptotic double-staining (D and F) assays showing no significant difference between Dt-MSC-exo and MSC-exo in the tolerance of BCs to Dox. Compared with nc-siRNA, treatment with S100A6 siRNAs resulted in more apoptotic cells in MSC-exo and Dt-MSC-exo. (C and D) MDA-MB-231 cells; (E and F) MCF-7 cells. The data are mean ± SD, averaged from three separate experiments. ∗p < 0.05, ∗∗p < 0.01. n.s., not significant.