Figure 6.

MSC-exo and Dt-MSC-exo Promote the Expression of S100A6 in BCs by Delivering miR-21-5p

(A and B) Relative expression of miR-21-5p in MSCs (A) and MSC-exo (B). The Dox-treated group displayed a higher content than did the control group. (C and D) MDA-MB-231 cells and MCF-7 cells were transfected with miR-21-5p antagomir or negative control scrambled RNA. Relative expression of miR-21-5p (C) and S100A6 (D) in MDA-MB-231 cells (left) and MCF-7 cells (right) treated with miR-21-5p antagomir. Two independent experiments showed similar results. (E and F) Reduced miR-21-5p expression in MSCs (E) and MSC-exo (F). (G) The mRNA and protein expression of S100A6 in MSC-exo and Dt-MSC-exo measured after treatment with miR-21-5p antagomir. Two independent experiments showed similar results. The data are mean ± SD, averaged from three separate experiments. ∗p < 0.05, ∗∗p < 0.01. n.s., not significant.