Skip to main content
. 2020 Nov 25;8(2):e000996. doi: 10.1136/jitc-2020-000996

Figure 4.

Figure 4

Combination of anti-PD1+IFNα is able to restore a well-activated immune infiltrate. Mice received 2 injections of either control (blue circles) or IFNα (light gray circles) or anti-PD1 (dark gray circles) or both (pink circles) in order to prevent tumor relapse at days 14 and 16 post first injection of E7 vaccine+IFNα. (A–C) Measurements were performed at day 17. (A) The proportion of CD45+ cells in tumors after the different treatments was determined by flow cytometry. n=8–15 mice from 3 to 5 independent experiments. Results are expressed as mean±SEM. Tukey’s multiple comparison test. *p<0.05; **p<0.01; ***p<0.001. (B) The proportion of CD8 T cells, tumor-associated macrophages (TAM) and monocytes among that CD45+ are shown. n=20–40 mice from 5 independent experiments. Results are expressed as mean±SEM. Tukey’s multiple comparison test. *p<0.05; **p<0.01; ***p<0.001. (C) The expression of PD1 on CD8 T cells and MHC class II on TAM and monocytes was determined in each condition. n=6–30 mice from 3 to 5 independent experiments. Results are expressed as mean±SEM. Tukey’s multiple comparison test. *p<0.05; **p<0.01; ***p<0.001.