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. 2020 Aug 19;11(1):249–272. doi: 10.1016/j.jcmgh.2020.08.003

Figure 10.

Figure 10

Autocrine/paracrine TNFSF15 promotes NOD2-induced antimicrobial pathways. (A–E) MDMs were treated with 100 μg/mL MDP for 48 hours ± neutralizing αDR3 antibodies (or isotype control). (A) ROS induction (n = 6; similar results in an additional n = 4). (B–E) Expression of the indicated proteins (n = 6; similar results in an additional n = 6). (F–I) MDMs were transfected with empty vector or vectors expressing p47phox, NOS2, ATG5, alone or in combination, and treated with 100 μg/mL MDP for 48 hours ± neutralizing αDR3 antibodies (1 hour pretreatment). (F) p47phox expression and ROS production. (G) NOS2 expression. (H) ATG5 and LC3II expression (n = 4, similar results in an additional n = 4). (I) Intracellular bacterial clearance (n = 8 from 2 independent experiments). Means + SEM. (A–E) Significance is between isotype control and neutralizing αDR3 antibodies for MDP-treated cells. ∗∗P < .01; ∗∗∗P < .001; †P < 1 × 10-4; ††P < 1 × 10-5. CFU, colony-forming unit; MFI, mean fluorescence intensity; Tx, treatment.