Skip to main content
. 2020 Aug 19;11(1):249–272. doi: 10.1016/j.jcmgh.2020.08.003

Figure 12.

Figure 12

TNFSF15 signaling potentiates NOD2-mediated intracellular bacterial clearance and autocrine/paracrine TNFSF15 promotes bacterial clearance upon stimulation of multiple PRRs. (A and B) MDMs were pretreated with 100 μg/mL MDP and 24 hours later treated with 10 ng/mL TNFSF15 for an additional 48 hours. (A) Timeline schematic for treatment strategy. (B) Intracellular bacterial clearance was assessed (colony-forming units [CFU]) (n = 12 from 2 independent experiments). (C) MDMs were treated with 10 μg/mL Pam3Cys, 0.1 μg/mL lipid A, or 10 μg/mL CpG for 48 hours ± neutralizing αDR3 antibodies (1 hour pretreatment) (or isotype control), then co-cultured with the indicated bacteria, and intracellular bacterial clearance was assessed (colony-forming units [CFU]) (n = 12 from 2 independent experiments). Significance is between isotype control to neutralizing αDR3 antibodies for the corresponding treatment condition or as indicated. Means + SEM. †P < 1 × 10-4; ††P < 1 × 10-5.