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. 2020 Aug 19;11(1):249–272. doi: 10.1016/j.jcmgh.2020.08.003

Figure 7.

Figure 7

The TRAF2/RIP1/RIP3 pathway is required for optimal TNFSF15-induced MAPK and NF-κB signaling. (A and B) MDMs were transfected with scrambled, a combination of ERK/p38/JNK (MAPKs), or NEMO siRNA, and then treated with 10 ng/mL TNFSF15. Fold mRNA expression at 4 and 48 hours (n = 6, similar results in an additional n = 6). (C and D) MDMs were transfected with scrambled, TRADD, combined FADD/MALT1/caspase-8 (F/M/C), or combined TRAF2/RIP1/RIP3 (T/R1/R3) siRNA, and then treated with 10 ng/mL TNFSF15. (C) Fold phospho-protein expression at 15 minutes (n = 8 from 2 independent experiments). (D) Fold phospho-protein expression at the indicated times (n = 8 from 2 independent experiments). Means + SEM. Significance is between scrambled and the target siRNA for the corresponding TNFSF15-treated cells or as indicated. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; †P < 1 × 10-4; ††P < 1 ×10-5. Scr, scrambled; Tx, treatment.