Figure 2.

Novel object test for object memory. A novel object test was used to assess memory in each dietary condition by comparing percent time the mouse spends with a new object vs. an old object. Three litters of mice were used per condition with multiple litter mates averaged to represent one data point. Five-month-old folic acid (FA) litters contained 4 mice each and FA-deficient (DEF) litters contained 3 mice each. Each 17-month-old litter contained 3 mice. All comparisons represent P ≤ 0.03 (noted by astrisks). Error bars represent SEM values calculated from the independent biological replicates across all litters.